Proteolysis by Streptococcus Lactis
نویسندگان
چکیده
منابع مشابه
Autolysis of Lactococcus lactis is influenced by proteolysis.
The autolysin AcmA of Lactococcus lactis was shown to be degraded by the extracellular lactococcal proteinase PrtP. Autolysis, as evidenced by reduction in optical density of a stationary-phase culture and concomitant release of intracellular proteins, was greatly reduced when L. lactis MG1363 cells expressed the cell wall-anchored lactococcal proteinase PrtP of the PI-type caseinolytic specifi...
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The kinetic properties of pyruvate kinase (ATP:pyruvate-phosphotransferase, EC 2.7.1.40) from Streptococcus lactis have been investigated. Positive homotropic kinetics were observed with phosphoenolpyruvate and adenosine 5'-diphosphate, resulting in a sigmoid relationship between reaction velocity and substrate concentrations. This relationship was abolished with an excess of the heterotropic e...
متن کاملExtracellular Proteinase of Streptococcus Lactis.
Williamson, W. T. (North Carolina State of the University of North Carolina, Raleigh), S. B. Tove, and M. L. Speck. Extracellular proteinase of Streptococcus lactis. J. Bacteriol. 87:49-53. 1964.-Streptococcus lactis was shown to produce an extracellular proteolytic enzyme(s). A 120-fold purification of the proteinase was obtained from the cell-free culture medium by ammonium sulfate fractionat...
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Polyethylene glycol-treated protoplasts prepared from Streptococcus lactis LM3302, a lactose-negative (Lac) derivative of S. lactis ML3, were transformed to lactose-fermenting ability by a transductionally shortened plasmid (pLM2103) coding for lactose utilization.
متن کاملHigh-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA.
Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those described for Bacillus subtilis (G. Chang and S. N. Cohen, Mol. Gen. Genet. 168:111-115, 1979).
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1929
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(29)93564-5